Map distance from the gene to centromere = # of MII asci/2 x 100
  total # of asci

 

Linked genes: PD asci= NPD asci
  NPD asci / Tetratype asci > 1/4

 

Unlinked genes: PD asci >> NPD asci
  NPD asci / Tetratype asci <1/4

 

Map distance between 2 genes: (# of Tetratype asci / 2) + (# of NPD asci) x 100
  total # of asci