In the calibration curve procedure, the signal from one or more calibration standards is measured and compared directly to the signal measured for the sample(s). Usually the calibration standards are fairly simple: for example, solutions will often contain the analyte as the only solute. The calibration curve method is most useful when matrix effects on the signal are absent.

The following topics are covered sequentially in this tutorial. You may click on a link to go directly that topic; otherwise use the arrows to move through the tutorial.

• methods development: choosing calibration standard concentrations
• quick and dirty: one- and two-point calibration curves
• linear calibration curves - general procedure
• potentiometric analysis using calibration curves
• nonhomogeneous noise: the weighted least-squares procedure
• using nonlinear calibration curves to estimate analyte concentration